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airyscan joint deconvolution processing module  (Carl Zeiss)


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    Structured Review

    Carl Zeiss airyscan joint deconvolution processing module
    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an <t>Airyscan</t> detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.
    Airyscan Joint Deconvolution Processing Module, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/airyscan+processing/ZEN+Module+Deconvolution/pmc13043941-504-8-17
    Average 94 stars, based on 42 article reviews
    airyscan joint deconvolution processing module - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Plakoglobin transmits tension across VE-cadherin for vascular leak formation and leukocyte diapedesis"

    Article Title: Plakoglobin transmits tension across VE-cadherin for vascular leak formation and leukocyte diapedesis

    Journal: The EMBO Journal

    doi: 10.1038/s44318-026-00732-0

    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.
    Figure Legend Snippet: ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.

    Techniques Used: Staining, Microscopy

    Related Articles

    Software:

    Article Title: Mechanisms of vascular damage by systemic dissemination of the oral pathogen Porphyromonas gingivalis
    Article Snippet: Chamber slides were analysed using a Zeiss LSM 880 invert Axio Imager and AiryScan confocal microscope. .. Images were processed using AiryScan processing (Zeiss, Oberkochen, Germany) and composite and orthogonal views were created using FIJI software (National Institute of Health, Bethesda, USA). ..

    Article Title: Phosphatidic acid drives spatiotemporal distribution of Pex30 at ER-LD contact sites.
    Article Snippet: .. Airyscan images were processed by Airyscan processing using the Zeiss ZEN software package. ..

    Article Title: LYST deficiency impairs autophagic lysosome reformation in neurons and alters lysosome number and size
    Article Snippet: Super-resolution live imaging was performed using Zeiss LSM 880 microscope equipped with a 63x/1.4 numerical aperture (NA) objective. .. Super-resolution imaging was performed using the Airyscan mode and the images were processed using Airyscan processing in ZEISS ZEN microscope software. ..

    Imaging:

    Article Title: LYST deficiency impairs autophagic lysosome reformation in neurons and alters lysosome number and size
    Article Snippet: Super-resolution live imaging was performed using Zeiss LSM 880 microscope equipped with a 63x/1.4 numerical aperture (NA) objective. .. Super-resolution imaging was performed using the Airyscan mode and the images were processed using Airyscan processing in ZEISS ZEN microscope software. ..

    Microscopy:

    Article Title: LYST deficiency impairs autophagic lysosome reformation in neurons and alters lysosome number and size
    Article Snippet: Super-resolution live imaging was performed using Zeiss LSM 880 microscope equipped with a 63x/1.4 numerical aperture (NA) objective. .. Super-resolution imaging was performed using the Airyscan mode and the images were processed using Airyscan processing in ZEISS ZEN microscope software. ..

    Article Title: Glia Cell Morphology Analysis Using the Fiji GliaMorph Toolkit.
    Article Snippet: .. As AiryScan microscopy and processing are specific to the microscope with which the data were acquired, the reader is referred to perform AiryScan processing [noniterative linear Wiener deconvolution algorithm (Huff, 2015; Zeiss, 2019) as indicated by their microscope manual]. ..



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    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an <t>Airyscan</t> detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.
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    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an <t>Airyscan</t> detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.
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    Image Search Results


    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.

    Journal: The EMBO Journal

    Article Title: Plakoglobin transmits tension across VE-cadherin for vascular leak formation and leukocyte diapedesis

    doi: 10.1038/s44318-026-00732-0

    Figure Lengend Snippet: ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.

    Article Snippet: Zeiss LSM 980 Airyscan acquisitions were processed with Airyscan Joint Deconvolution processing module in ZEN blue software (Zeiss) to improve signal-to-noise and spatial resolution; LSM 880 images were used without further deconvolution.

    Techniques: Staining, Microscopy